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Discovery Services

In vitro biological evaluation platform

Services
Hit Compound Discovery

Method Development

High-Throughput Screening

Hit Compound Discovery

Lead Optimization

Method Optimization

In Vitro Target Binding Experiments

In Vitro Functional Activity Experiments

Disease Model Establishment

Mechanism of Action Research

Cell Experiments mainly to Verify Targets

Method Research, Development, and Optimization

Enzyme and Cell Screening

Further Functional Verification Based on Cell Biology, Immunology, Signal Pathways, etc., FACS, qPCR, AlphaLISA, ELISA, Proliferation Experiments, etc.

Main Target Types

Target Types

Signal Pathway

GPCR

GTPγS (Non-Radiometric GTPγS Assays)

cAMP (HTRF; agonist/antagonist)

Calcium flux (Facs; IP-one)

β-arrestin (Tango; PathHunter)

Cellsensor (NFAT-bla; CRE-bla)

Enzyme

Binding (Filtration; lantha, TDF)

ADP quantitation (ADP-Glo, etc.)

Phospho-substrate detection (HTRF, IMAP, Lantha screening, EZ reader, LC/MS/MS)

Cellular phospho-protein detection (AlphaScreen, MSD, HCS, WB)

Immuno-Oncology

Checkpoint blockade both biochemical Binding and cellular reporter assays

In-house co-culture reporter assays

Whole blood, PBMC, T cell, DC, B and NK cellular assays available

Ex vivo assays MLR, co-culture, etc

Cytokine release assays FACS, ELISA

Ion channel

Binding (FP based binding)

Electrophysiological functional assay (manual patch-clamp, automated patch-clamp)

Fluorescence functional assay (FACS)

Nuclear Receptor

Reporter gene-based assay (transient transfection, stable cell line)

FP based binding assay TaqMan based gene expression

Cell line engineering

Stable overexpression (Flip-In, lentiviral and Retroviral system, Neon electroporation, T-REx system, HiBiT and PPI)

Reporter, mutant, tag and tag-free expression

Knock-out (KO) and Knock-In (KI) by CRISPR-Cas 9

Knock-down (KD) study by siRNA, shRNA and CRIPSR-Cas9

Experimental Methods

Experiments

Methods

Cellular Experiments

Cell Proliferation (2D, 3D, long-term (HTP), short-term conditions)

Cell Viability Test (reagents such as CTG, CyQunat, CCK-8, MTT)

Cell Migration and Invasion

Nuclear Transport Experiments

Apoptosis Experiment

Cell Cycle

Cell Colony Experiments

Angiogenesis

Cell Line Engineering

High-Content Platform

WB

qPCR

ELISA

Immuno-oncology Related Experiments

Immune cell isolation and culture, such as PBMC, T, B, NK, DC, and other cells

Antibody Functional Evaluation，ADCC,ADCP，CDC， Co-Culture, MLR assays

Innate and Acquired Immune Related Pathway Activity Test

Immune cell activation, proliferation, differentiation

Cytokine Detection

Flow Cytometry Analysis

Immune cell typing

Immune checkpoint activity test

Tumor Killing Experiment

Tumor Infiltration Experiment

Enzyme Experiments

Kniase assays

GPCR related methods

Protein interaction

Methods: HTRF, ADP-Glo, Caliper, ELISA, Alphascreen, etc

Other enzyme targets, such as phosphatase, protease, ubiquitin ligase, metabolic enzyme classes

Enzyme method development and optimization

Phenotypic

and DNA Damage

Protein acetylation

Protein methylation

mRNA modification

DNA damage model

DNA exonuclease

RNA enzyme

Reverse transcriptase

Protein enzyme

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Discovery Services

In vitro biological evaluation platform

Target Types	Signal Pathway
GPCR	GTPγS (Non-Radiometric GTPγS Assays) cAMP (HTRF; agonist/antagonist) Calcium flux (Facs; IP-one) β-arrestin (Tango; PathHunter) Cellsensor (NFAT-bla; CRE-bla)
Enzyme	Binding (Filtration; lantha, TDF) ADP quantitation (ADP-Glo, etc.) Phospho-substrate detection (HTRF, IMAP, Lantha screening, EZ reader, LC/MS/MS) Cellular phospho-protein detection (AlphaScreen, MSD, HCS, WB)
Immuno-Oncology	Checkpoint blockade both biochemical Binding and cellular reporter assays In-house co-culture reporter assays Whole blood, PBMC, T cell, DC, B and NK cellular assays available *Ex vivo* assays MLR, co-culture, etc Cytokine release assays FACS, ELISA
Ion channel	Binding (FP based binding) Electrophysiological functional assay (manual patch-clamp, automated patch-clamp) Fluorescence functional assay (FACS)
Nuclear Receptor	Reporter gene-based assay (transient transfection, stable cell line) FP based binding assay TaqMan based gene expression
Cell line engineering	Stable overexpression (Flip-In, lentiviral and Retroviral system, Neon electroporation, T-REx system, HiBiT and PPI) Reporter, mutant, tag and tag-free expression Knock-out (KO) and Knock-In (KI) by CRISPR-Cas 9 Knock-down (KD) study by siRNA, shRNA and CRIPSR-Cas9

Experiments	Methods
Cellular Experiments	Cell Proliferation (2D, 3D, long-term (HTP), short-term conditions)
	Cell Viability Test (reagents such as CTG, CyQunat, CCK-8, MTT)
	Cell Migration and Invasion		Nuclear Transport Experiments
	Apoptosis Experiment	Cell Cycle	Cell Colony Experiments
	Angiogenesis	Cell Line Engineering	High-Content Platform
	WB	qPCR	ELISA
Immuno-oncology Related Experiments	Immune cell isolation and culture, such as PBMC, T, B, NK, DC, and other cells
	Antibody Functional Evaluation，ADCC,ADCP，CDC， Co-Culture, MLR assays
	Innate and Acquired Immune Related Pathway Activity Test
	Immune cell activation, proliferation, differentiation
	Cytokine Detection	Flow Cytometry Analysis	Immune cell typing
	Immune checkpoint activity test	Tumor Killing Experiment	Tumor Infiltration Experiment
Enzyme Experiments	Kniase assays	GPCR related methods	Protein interaction
	Methods: HTRF, ADP-Glo, Caliper, ELISA, Alphascreen, etc
	Other enzyme targets, such as phosphatase, protease, ubiquitin ligase, metabolic enzyme classes
	Enzyme method development and optimization
Phenotypic and DNA Damage	Protein acetylation	Protein methylation	mRNA modification
	DNA damage model	DNA exonuclease	RNA enzyme
	Reverse transcriptase	Protein enzyme